Corrinoid coenzyme requirement for effective TCE bioremediation using Dehalococcoides
Microorganisms of the Dehalococcoides (Dhc) genus play a crucial role in remediating groundwater contaminated by chlorinated solvents. Their unique metabolic ability to convert chlorinated solvents such as per- and tri-chloroethene to innocuous end product ethene is attributed to corrinoid-dependent reductive dehalogenases. Corrinoids, the required coenzymes for reductive dehalogenase activity, are a group of cobalt-containing molecules (including vitamin B12) that have wide structural variability in the lower axial ligand. The currently known Dhcstrains cannot synthesize corrinoids de novo and thus have to obtain them from the environment. It has been reported that corrinoid limitation, which can impact the robustness and efficiency of dechlorination, can be avoided either by adding exogenous vitamin B12 or by growing Dhcin microbial consortia containing microorganisms capable of corrinoid synthesis.
Currently, little is known about the actual corrinoid species that Dhcobtains from their environment and needs to support their physiological functions. The goal of this study is to investigate both the corrinoid forms present in effective TCE-dechlorinating microbial communities and the structural specificity of the corrinoid coenzymes required by Dhc. The corrinoid forms and concentrations are being determined in the effective TCE-dechlorinating microbial communities that have been enriched with no external B12 amendment. The Dhc physiological responses to these corrinoid forms are being characterized. This study contributes both to our understanding of the corrinoid syntrophic interactions in effective TCE-dechlorinating microbial communities and to the structure-function relationship of corrinoid coenzymes on the metabolic fitness of Dhc.
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Oxygenase-Catalyzed Biodegradation
of Emerging Water Contaminants: 1,4-Dioxane and N-Nitrosodimethylamine
Quantifying Gene Expression to Predict and Optimize Reductive Dechlorination by Dehalococcoides spp.
Application of Microarrays to Identify Biomarkers of Reductive Dehalogenating-Microbial Communities
Using Molecular and Isotopic Tools to Characterize the Biodegradation of Chlorinated Ethenes and Ethanes
Characterizing the fate and biotransformation of fluorochemicals in aqueous film forming forms (AFFF)
Corrinoid coenzyme requirement for effective TCE bioremediation using Dehalococcoides
Meta-omics of Microbial Communities Involved in Bioremediation
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